Neurons in the brainstem promote REM sleep and trigger brainwaves that might cause dreaming

Dr. Amanda (Mandy) Schott was the lead author on this study. As a researcher, Mandy is most interested in defining neural circuits, and how specific populations of cells communicate to generate essential human behaviors such as sleep.

or technically,

A medullary hub for controlling REM sleep and pontine waves

[See Original Abstract on Pubmed]

Authors of the study: Amanda Schott, Justin Baik, Shinjae Chung & Franz Weber

Rapid eye movement (REM) sleep is the sleep state that most people associate with dreaming, however REM sleep has many other essential functions. While REM makes up only about 20-25% of our nightly sleep, it is vitally important for memory, emotional processing, and other functions we have yet to understand. This is true not just for humans, but all mammals and maybe even birds and reptiles! To facilitate all these functions of REM, the brain is highly active during this sleep state. In fact, during REM sleep, brain signals look more similar to wake than non-REM sleep. Because of this, REM sleep is sometimes called paradoxical sleep because paradoxically, the brain is so active during rest. 

Surprisingly, we still know very little about how the brain switches from low-activity non-REM sleep to high-activity REM sleep. Moreover, during REM sleep there are sometimes sporadic brain waves that seem to be important for normal brain function but whose precise role is still not totally clear. P-waves are one such waveform that is caused by lots of synchronous neuronal activity in the back of the brain, in a brainstem region called the pons. From the pons, P-waves travel forwards in the brain to brain regions important for forming and storing memories, and also areas involved in visual processing. These P-waves are interesting because they occur only during REM sleep, and are proposed to be involved in dreaming and the memory functions of REM sleep. A paper by recent NGG graduate Dr. Amanda Schott investigated two major unknowns in REM sleep research: 1) What neurons and brain regions are involved in generating REM sleep, and 2) What neurons and brain regions are involved in generating P-waves. Is it possible that one set of neurons could do both? 

While we know of several brain regions in the brainstem that regulate REM sleep, most of them consist of inhibitory neurons, meaning they “turn off’ other brain regions to promote REM sleep. Dr. Schott, however, found a highly unusual group of excitatory neurons in part of the brainstem called the dorsal medial medulla (dmM). These excitatory neurons can “turn-on” other neurons they make connections with. These dmM excitatory neurons were only active during REM sleep, suggesting they may be involved in  promoting REMs sleep. In addition, dmM neurons project their axons and send signals to the part of the pons that is known to generate p-waves. In fact, the dmM neurons were active at the same time the p-waves occurred suggesting that the dmM excitatory neurons could be involved in the generation of p-waves too! Dr. Schott next wanted to directly manipulate the activity of these neurons to see if they could cause transitions to REM sleep or cause generate p-waves. 

Using a modern neuroscience technique called optogenetics, Dr. Schott was able to cause the neurons in the dmM to fire when a laser light was shined over them through an optic fiber. She simultaneously determined if the mouse was awake, asleep, or in REM sleep by measuring the mouse’s brain waves using electroencephalography, or EEG. She found that stimulating these neurons caused the mouse to enter REM sleep, and also increased the length of REM sleep episodes. Shining the laser light also caused a p-wave to be generated when the light was shined about 60-100% of the time when the mouse was sleeping. Experimentally reducing the activity of the dmM neurons also decreased the amount of REM sleep, as well as the amount of p-waves. Dr. Schott interpreted these findings as evidence that dmM excitatory neurons are critical for normal amounts of REM sleep to occur, and for triggering p-waves. 

Overall, Dr. Shott’s work adds an important piece to the puzzle to our understanding of which brain regions can promote REM sleep. Her findings are an important first step in understanding which neurons generate p-waves which is ultimately necessary to understand p-wave function. This work will provide a foundation on which others (including the author of this piece!) can study the role of p-waves in REM sleep, and move closer to finally understanding how and why we dream.

About the brief writer: Emily Pickup

Emily is a 4th year PhD candidate in Dr. Franz Weber’s lab. She is interested in the biological functions of sleep. Specifically, she is interested in understanding the function of REM-specific p-waves. The large pontine waveform implicated in memory consolidation discussed in the brief above.

Interested in learning more about REM sleep and p-waves? See the original paper here.

A mouse model for autism and ADHD can mimic sex differences in sleep

or technically,

Hyperactivity and male-specific sleep deficits in the 16p11.2 deletion mouse model of autism.

[See Original Abstract on Pubmed]

Authors of the study: Angelakos CC, Watson AJ, O'Brien WT, Krainock KS, Nickl-Jockschat T, Abel T.

Falling asleep at night is something we look forward to at the end of the day, restoring our energy for the new day ahead. However, a good night's rest isn’t guaranteed for everyone as many people across the country have trouble sleeping. This is especially true for people diagnosed with autism spectrum disorders (ASD) and attention deficit-hyperactivity disorder (ADHD) who often have trouble falling asleep and staying asleep. Up to 80% of individuals diagnosed with ASD and 55% of children with ADHD suffer from sleep problems. Sleep disturbances can worsen other symptoms common in these disorders such as repetitive behaviors, attention, and communication. Christopher Angelakos, a graduate student in Dr. Ted Abel’s lab, wanted to understand why sleep disturbances are common in ASD and ADHD. In order to answer this question, Christopher turned to established models of ASD/ADHD. He reasoned that mice that have ASD/ADHD-like symptoms might also have sleep disturbances.

Patients with disorders like ASD/ADHD often have changes in the number of copies they have for a geneA unit of DNA that encodes a protein and tells a cell how to function. Typically, for each geneA unit of DNA that encodes a protein and tells a cell how to function there are two copies - one from each parent. Therefore, individuals with ASD/ADHD can have more copies, or fewer copies (also known as a deletion). One of these changes is a deletion in chromosomal region 16p11.2. People that have a deletion in this region are more likely to have ASD and ADHD. Previous research has shown that mice with a deletion in the 16p11.2 region show symptoms similar to ASD/ADHD like differences in brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. structure, cognitive ability, and communication. However, sleep problems remained largely unexplored, a problem that Christopher wanted to address.

Christopher observed that these animals were hyperactive, a behavior that is observed in individuals diagnosed with ADHD. He tracked all of the movements of the mice in their cages, observing an increase in activity in the 16p11.2 deletion mice throughout the day, and a robust increase during the dark (active) phase of their cycle. This led him to think that something may be altered in their circadian rhythms. To investigate this he monitored them for 24hrs and measured their sleep and activity to determine if it was normal.

He also examined their sleep cycles using polysomnography, which tracks brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. waves, eye movements, and limb movement during sleep. He wanted to know whether this was a problem of initiating sleep or maintaining sleep. He found that once the animals were asleep, they usually remained asleep for the same amount of time indicating that there was not a problem of staying asleep. On the other hand, once an animal was awake, it was usually awake for a longer period of time, indicating that it may have had trouble with initiating sleep. When Christopher further analyzed the data, he saw male mice with the 16p11.2 deletion spent a longer amount of time awake than regular mice. Coupled with his finding that these mice stay asleep as long as the regular mice, this suggests that they had a hard time falling asleep, rather than that they were waking up multiple times and having brief amounts of wakefulness. Interestingly, these disorders are more commonly found in males rather than females. Males are four times more likely to be diagnosed with ASD and three times more likely to be diagnosed with ADHD.

Issues with sleep in people that are diagnosed with autism or ADHD is a problem that needs to be addressed. Christopher asked whether we can use a mouse to model sleep problems in autism? He showed in his paper that the 16p11.2 deletion mouse can model sleep disturbances that are seen in humans. He is excited to see future work using this mouse model to uncover specific brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. circuits that may be involved and better treatment for sleep problems. Now that we have this experimental mode, we can determine if improving sleep quality will improve other psychiatric symptoms.

About the brief writer: Felicia Davatolhagh

Felicia is a PhD Candidate in Marc Fuccillo’s lab. She is a seventh year studying the impact of neuropsychiatric disease on synaptic connectivity and synaptic function.

Want to learn more about sex differences in neurodevelopmental disorders? You can read Christopher’s whole paper here.

How support cells in the brain support sleep.

or technically,

Endocytosis at the Drosophila blood-brain barrier as a function for sleep.

[See Original Abstract on Pubmed]

Authors of the study: Gregory Artiushin, Shirley L Zhang, Hervé Tricoire, Amita Sehgal

Almost every day of our lives we close our eyes, count a few sheep, and drift off to sleep. If we don’t get enough sleep, our brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. doesn’t work as well and its normal functions, such as attention and decision-making, are affected. But why does this happen? Why is sleep so important? Although not everything about sleep is known, what scientists do know is that sleep is necessary to maintain the health of the cells in our body, especially the cells in our brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals.. NeuronsA nerve cell that uses electrical and chemical signals to send information to other cells including other neurons and muscles perform essential processes during sleep that they can’t do as efficiently while awake, such as consolidating memories and clearing waste. For this reason, sleep deprivation has serious consequences including build-up of toxic proteins¹. Therefore, neuroscientists have been curious about the mechanisms of sleep for quite some time. Gregory Artiushin, a UPenn neuroscience graduate student in the lab of Amita Sehgal, was interested in understanding this sleep process a little deeper, specifically thinking about how different cells in the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. may contribute to sleep function.

Although neuronsA nerve cell that uses electrical and chemical signals to send information to other cells including other neurons and muscles are important in mediating sleep, the non-neuronal support cells of the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. (known as glial cells) have also been linked to sleep regulation. Glial cells are a class of cells that surround all neuronsA nerve cell that uses electrical and chemical signals to send information to other cells including other neurons and muscles and are critical for their survival; they perform important ‘maintenance’ tasks for neuronsA nerve cell that uses electrical and chemical signals to send information to other cells including other neurons and muscles including providing them with nutrients and oxygen, insulating their electrical connections, and clearing dead cells and waste from their surroundings. Glial cells may help with waste clearance in the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. during sleep, and can also release molecules that promote sleep². In order to carry out their functions properly, glial cells have to move cargo into and out of the cell. This is mainly done through endocytosis, where things outside of the cell are captured into sacs and brought into the cell, much like packaging something important for transport. Although this process of endocytosis is important for glial cell performance overall, scientists still aren’t sure if endocytosis in glial cells is important for sleep. Additionally, it is not known which of the many types of glial cells are important in regulating sleep (there are over four main classes of glia).

Greg decided to use fruit flies to study the importance of endocytosis in sleep. Yes, flies sleep too! Not only are their sleeping patterns similar to that of humans, with a long period of sleep at night, but their genesA unit of DNA that encodes a protein and tells a cell how to function can also be easily manipulated in order to help scientists establish which genesA unit of DNA that encodes a protein and tells a cell how to function are important in regulating sleep. About 75% of known human disease genesA unit of DNA that encodes a protein and tells a cell how to function have a recognizable match in the genetic code of fruit flies³. These qualities make them a popular ‘model’ amongst scientists for studying sleep and its underlying mechanisms. To understand how endocytosis changed with increased sleep need, Greg deprived flies of sleep and then looked at how endocytosis was affected in their glial cells. He found that endocytosis was increased after sleep deprivation, and that this correlated with how sleep-deprived the flies were. Since this suggested that endocytosis was somehow linked to sleep, Greg wanted to explore this link further by blocking endocytosis entirely and seeing what happened to sleep. To do this, he generated a mutated form of a geneA unit of DNA that encodes a protein and tells a cell how to function that is critical for endocytosis in flies, allowing him to effectively block endocytosis in these animals. By mutating this geneA unit of DNA that encodes a protein and tells a cell how to function only in glial cells, Greg was able to block endocytosis exclusively in glial cells of the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals.. Interestingly, he saw that this increased how long the flies slept, suggesting that endocytosis in glia somehow controls the process of sleep.

Since there are many types of glial cells, Greg wanted to next understand which type of glial cells were important in sleep. Using the same genetic mutation strategy, Greg blocked endocytosis in each specific type of glial cell: he expressed the mutation in one type of glial cell at a time while leaving endocytosis in all of the other types of glia intact. This allowed him to determine which type of glial cell(s) was responsible for the effects he saw when he blocked endocytosis in all glial cells. He found that endocytosis in one particular type of glial cell was linked to sleep duration. This type of glial cell makes up the blood-brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. barrier in flies. The blood-brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. barrier, or BBB, is composed of tightly-linked glial cells that separate the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. from the rest of the body. This barrier acts as a roadblock that prevents many substances from getting into the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals., which is crucial for protecting the brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. from pathogens or toxins. Greg found that blocking endocytosis only in the BBB glial cells caused changes in the structure of the barrier and increased sleep. However, blocking endocytosis in other types of glia did not affect the BBB or sleep.

Greg’s work suggests that endocytosis in the glial cells of the BBB of the fly is an important regulator of sleep, identifying a specific mechanism that may also be crucial in human sleep. Exactly how endocytosis at the BBB affects sleep duration remains unknown, but it is possible that this process may be important in waste clearance or maintenance of the BBB. If endocytosis is disrupted, these processes may be impaired leading us to sleep longer as a way of compensating. Future studies will aim to address why disrupting endocytosis in these BBB glial cells messes up the sleep cycle. Greg’s findings in this study (and future experiments) are important because they allow researchers to understand exactly how these processes at the BBB could be important for human brainThe brain is an organ that serves as the center of the nervous system in all vertebrate and most invertebrate animals. function, and how they may be altered in sleep deprivation and sleep disorders, such as insomnia. Scientists might finally be on track to figure out why pulling an all-nighter turns us into sleep-deprived zombies!

About the brief writer: Claudia Lopez

Claudia is a fourth year Neuroscience graduate student studying HIV-related neurodegeneration. She uses cell culture system to study how HIV infection leads to neuronal dysfunction.

Citations:

1. Shokri-Kojori E, Wang G, Wier CE, Demiral SB, Guo M, Kim SW . . . Volkow ND. (2018). β-Amyloid accumulation in the human brain after one night of sleep deprivation. Proceedings of the National Academy of Sciences, 115(17): 4483-4488. You can find the paper here.

2. Halassa MM, Florian C, Fellin T, Munoz JR, Lee S, Abel T . . . Frank MG. (2009). Astrocytic modulation of sleep homeostasis and cognitive consequences of sleep loss. Neuron, 61(2): 213-219. You can find the paper here.

3. Pandey UB & Nichols CD. (2001). Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery. Pharmacological Reviews, 11(6): 1114-1125. You can find the paper here.